The Accuri C6 Flow Cytometer does not produce aerosols during normal operation. Higher concentrations might result in inaccurate counting due to system saturation, so dilute samples if necessary. BD Accuri C6 Plus fluorochrome support All fluorochromes in the table can be detected by the BD Accuri C6 Plus equipped with standard filters. Click on the Windows Start button and select Control Panel from the pop-up menu. The BD Accuri ® C6 is a digital basic flow cytometer, which is equipped with a Blue (488nm) and a Red (640nm) lasers, two light scatter detectors (FSC and SSC), and four fluorescence detectors. Clinical Research and the Rockefeller University Hospital, Chemers Neustein Summer Undergraduate Research Fellowship Program, Experience Science, the Arts, and Culture. A unique, non-pressurized, peristaltic pump system drives the fluidics. A sheath-focused core enables event rates of up to 10,000 events per second and a sample concentration of over 5 x 106 cells per mL. hެ�mS�8ǿ�^��[���L��@H�]�LC�w���$J�c����O�R�B ��:aGZ�V��6h���eZ3.ӆ !�N��Ӗ).�Θ����Y�2��V2#W�%㙱�(&���h&2�OäV 3��b#�(!5{���W�֕78���L�/��� Gը�_���~=ZU0�-a��aPW�Cw�����W8v4 �•���r���֍qlj�Į��!��e���N�f��p�c��ޟ-[ޟ�0��͵��I�~F�������ѭ3��w0�C�z�����?~������S�d��r��>U�j���~1���U#�^�&L�˥ӥM�����R)拷���. the Accuri C6 Flow Cytometer System. 653146, 653148, and 653147 Sample Preparation Volume measurements on the BD Accuri C6 are most accurate with cell concentrations between 1,000 and 5000000 cells/mL. BD LSRFortessa X-20. Hi, our Accuri C6 has stopped taking liquid. All rights reserved. The default configuration of the BD Accuri ® C6 is similar to the FACSCalibur-2 (3-Blue 1-Red) with filters optimized for the detection of fluorochromes such as FITC, PE, PerCP, and APC. 661085). BD Accuri™ C6 software was started. Enjoy the videos and music you love, upload original content, and share it all with friends, family, and the world on YouTube. Since yeasts are relatively large (I use an Accuri C6 to count bacteria so yeast are large to me) I would go with the faster/larger core size. Optional filters can be used to increase resolution or to separate fluorochromes with overlapping signals, like GFP and YFP. Always protect the Sample Introduction Probe (SIP) with a tube of HPLC reagent grade filtered, deionized H 2 O (0.22 µm filter), or equivalent, when the cytometer is not running samples. The non-pressurized system of BD Accuri C6 supports any brand of sample tubes – 12 x 75 mm or smaller (including microcentrifuge tubes), made of polypropylene or polystyrene. The BD CSampler Plus adds a minimal footprint to the BD Accuri C6 Plus, about three feet square for the pair, keeping your benchtop free for other uses. BD Accuri™ C6 Flow Cytometer Features: Equipped with a blue and a red laser, two light scatter detectors, and four fluorescence detectors with optical filters optimized for the detection of fluorochromes such as FITC, PE, PerCP, and APC. BD LSRFortessa 1. BD Accuri C6, C6 Plus, and BD FACSVia Cytometers Perform these procedures when starting up your instrument after an extended period. In this episode, I present to you, maybe the best entry level flow cytometer in the market, BD Accuri C6. %%EOF Such wear includes scratches, scrapes, and general dirtiness. ... filters optimized for the detection of fluorochromes such as FITC, PE, PerCP, and APC. The BD Accuri C6 flow cytometer was switched on and allowed to warm up for 15 minutes before analyses were begun. Came with note that it needs repair. Available to trained users with online booking. -Ģ �r^�F)�?2��7�% �K��x4JR���E�Y��Wl������3� �;p�y[�AD= r 29 0 obj <> endobj BD LSRFortessa 2. Easy to use - fixed alignment and pre-optimized detector settings. Item is sold AS IS. The Selectable Lasers Module allows operation of the system in two additional configurations (2-Blue 2-Red, and 4-Blue) and significantly expands the fluorochrome combinations that can be analyzed. 3. Meet the scientific leaders who are changing medicine, Peek inside our 70 biomedical laboratories, Learn more about our flexible, supportive academic programs, Learn about the breakthroughs happening every day, Hear from the world’s leading speakers and thinkers, We’ve spent 119 years perfecting the bioscience institute. Even more colors to choose from with selectable lasers and filter replacement. endstream endobj startxref Table of Contents. Training can be requested via PPMS. BD offers a selction of inferior vena cava filter products. 1 The standard configuration of the BD Accuri C6 Plus (as shipped) is “3-blue 1-red” with the 533/30 (formerly 530/30) filter in FL1, 585/40 in FL2, 670 LP in FL3, and 675/25 in FL4. Performance validation of the BD Accuri C6 … Prevents the growth of bacteria in the sheath fluid of the Accuri™ C6 Flow Cytometer. The Accuri C6 cytometer in my lab has some problems, and we need some help. ... optical filters optimized for the detection of FITC, PE, PerCP, and APC. A compact optical design, fixed alignment, and pre- Filter and Fluorochrome List for the Accuri® C6 Flow Cytometer® System ©January, 2010 Standard Filters Optional Filters (other) Fluorescent Protein Filters (for simultaneous GFP/YFP detection) FL1 530 +/- 15nm FL1 510 +/- 7.5 nm 510 +/- 7.5 nm FL2 585 +/- 20 nm FL2 565 +/- 10 nm 540 +/- 15nm FL3 670nm LP FL3 610 +/- 10 nm 670nm LP C6 Flow Cytometer 1-year Maintenance Kit . 1230 York Avenue, Box 344 The default configuration of the BD Accuri® C6 is similar to the FACSCalibur-2 (3-Blue 1-Red) with filters optimized for the detection of fluorochromes such as FITC, PE, PerCP, and APC. You are viewing an Accuri C6 Flow Cytometer. - Find MSDS or SDS, a COA, data sheets and more information. The latest science discoveries delivered monthly to your inbox. Brand BD Accuri™ Contents Contains (12) Peristaltic Pump Tubing, (6) Sheath Bottle Filters, (12) Decon/Cleaner Bottle Filters and (6) In-Line Sheath Filters To increase signal resolution, or to separate fluorochromes with overlapping signals, a choice of optional, user-interchangeable filters is available. This item has been powered on, but no further testing. ... BD Accuri C6. Data is digitally collected over a 7-decade dynamic range (16 million channels of digital data), making all data available to users as needed. All reagents and kits are compatible with both the BD Accuri C6 Plus and BD Accuri C6 flow cytometer systems. Click on “View” tab 4. Brand: BD 653156 Phase III+: The University is open for expanded research operations; only authorized personnel will be admitted on campus. A compact optical design, fixed ... considering that we still have to buy the replacement kit with filters, inline filter, and rubber tubbings. Packaged in a pack of ten 8mL vials. h�b``�```�����`��π ���@q��L�n\g���`��h 'c�. Millex Sterile syringe filter units are available in a larger 33mm housing with either MCE, PVDF or PES membranes. BD Accuri® C6 CFlow SAMPLER supports the high throughput and fully automated acquisition from 48- and 96-well plates and deep-well plates. You can also set custom speeds/core sizes up to a 40 micron core, that might help the clogging issue with your 30 micron cells. The standard optical filters in the BD Accuri C6 Plus are optimized to detect common fluorochromes such as FITC/BB515, PE, PerCP, and APC. The BD Accuri C6 is a compact flow cytometer that uses a low-pressure pumping system to drive the fluidics allowing for the derivation of sample volume and calculation of absolute counts or sample concentration per microliter. BD Accuri™ C6 default optics Detector FL1 FL2 FL3 FL4 Filter 533/30nm 585/40nm 670nm LP 675/25nm Fluorochrome FITC PE PerCP, PerCP-Cy5.5 APC Color Green Yellow/Orange Dark Red Red “3 blue 1 … %PDF-1.6 %���� A compact optical design. Flow cytometry can rapidly quantitate bacteria and discriminate them from debris. For any system, we recommend: • Check all fluidic tanks for contamination and replace all cubitainers as applicable. Additional laser configurations (2-blue 2-red, 4-blue) are available with the Selectable Lasers Module (Cat. Which fluorochromes are compatible with the cytometers? 0 Accuri C6 is a digital PC based bench top analyser, 2 laser system. Amnis ImageStreamX. Rockefeller University The BD Accuri® C6 is a digital basic flow cytometer, which is equipped with a Blue (488nm) and a Red (640nm) lasers, two light scatter detectors (FSC and SSC), and four fluorescence detectors. It is also supplied with a 24-tube rack for the walk-away acquisition of standard 12 x 75-mm tubes. Access to training videos presented by FCR staff are here. New York, NY 10065. Designed from the ground up to be compact and easy to use, the BD Accuri C6 flow cytometer fits in any lab and is readily accessible Filter and Fluorochrome List for the Accuri C6 Flow Cytometer ® System As of: January 1, 2010 Standard Filters Optional Filters (other) Fluorescent Protein Filters (for simultaneous GFP/YFP detection) FL1 530 +/- 15nm FL1 510 +/- 7.5 nm 510 +/- 7.5 nm FL2 585 +/- 20 nm FL2 565 +/- 10 nm 540 +/- 15nm FL3 670nm LP FL3 610 +/- 10 nm 670nm LP In addition, the system derives sample volume and can calculate absolute counts or sample concentration per microliter. filter, and bottle filters. 52 0 obj <>/Filter/FlateDecode/ID[<6D17E613E92D6948AEF4F9A1572DFD51>]/Index[29 41]/Info 28 0 R/Length 106/Prev 60895/Root 30 0 R/Size 70/Type/XRef/W[1 2 1]>>stream endstream endobj 30 0 obj <>>> endobj 31 0 obj <> endobj 32 0 obj <>/ExtGState<>/Font<>/ProcSet[/PDF/Text]/Properties<>>>/Rotate 0/TrimBox[0.0 0.0 612.0 792.0]/Type/Page>> endobj 33 0 obj <>stream Light, rugged, and small enough to fit on a benchtop, it connects to standard electrical circuitry and can easily be transported to a water treatment facility or into the field. Researchers at the Swiss Federal Institute of Aquatic Science and Technology (Eawag, Switzerland) have developed a standard flow cytometric staining protocol and a corresponding BD AccuriTM C6 software analysis template to discriminate bacteria from debris in drinking water samples. Waste and sheath fluid bottles were checked during this time, and emptied or refilled as required. Nos. BD Accuri® C6 is manufactured with standardized fluorescence performance so that users do not need to adjust detector voltages. Online Booking System When we turn on, the system does not collect liquid as normally, in fact is completely silent (no peristaltic pump working). Select “Tools” from the menu bar. BD Accuri C6 Flow Cytometer Instrument Manual 7820018 Rev-4 1 1 INTRODUCTION The BD Accuri™ C6 flow cytometer offers an entirely new vision for the role of flow cytometry in life science research. The ideal cell concentration Cat. The BD Accuri C6 is a personal flow cytometer that brings flow cytometry within reach by being easy to use, simple to maintain, and affordable. The BD Accuri® C6 is a personal flow cytometer that brings flow cytometry within reach by being easy to use, simple to maintain, and affordable. Contact, Flow Cytometry Resource Center (FCRC) This Technical Note BD™ Accuri™ Bacteriostatic Concentrate Solution can be used to treat 10L of sheath fluid. Copyright 2004—2020 The Rockefeller University. Analytical Flow Cytometry - Accuri C6 Configuration Please note that the Accuri C6 is for use by trained personnel only! To view hidden file extensions which distinguish BD Accuri C6 Software files (.c6) from BD Accuri C6 Software template files (.c6t): 1. • Replace all fluid filters … filters. The BD Accuri C6 flow cytometer (Figure 2) offers performance, simplicity, and affordability. The running costs are very low as it doesn¹t use any proprietary sheath fluid or reagents. Ē\�@BBH"v@��d� �Hz �H4�0012����H%�?�ݏ x�S Overall very good cosmetic condition. With minor signs of normal wear and tear. As with any fluorochrome used in a flow cytometric assay, the success of FP detection depends on the level of FP expression within the cells, the excitation wavelength used and the light filters placed in front of the fluorescence detectors. 69 0 obj <>stream FCRC Waiting List Forms 2. No. Includes all accessories shown in pictures. The BD Accuri™ C6 is a personal flow cytometer that brings cell analysis within reach by being easy to use, simple to maintain, and affordable. Also the filters and lasers can be swapped out to suit your needs with easily available off-the-shelf substitutes (this was mentioned by one of the Accuri techs, I personally cannot verify this). h�bbd``b`:$� -�8�`�$XJA�H�H0'��U �.+ Standard fluorochrome in use are identical to FACS Calibur. 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